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高效的基因传递到多个中枢神经系统使用新界在子宫内电穿孔

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation

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高效的基因传递到多个中枢神经系统使用新界在子宫内电穿孔

DOI:

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13:12 min

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June 23, 2011

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Rajiv Dixit, Fuqu Lu, Robert Cantrup, Nicole Gruenig², Lisa Marie Langevin, Deborah M. Kurrasch, Carol Schuurmans

¹Department of Biochemistry and Molecular Biology, Hotchkiss Brain Institute, Alberta Children’s Hospital Research Institute,University of Calgary , ²Department of Medical Genetics, Alberta Children’s Hospital Research Institute, Hotchkiss Brain Institute,University of Calgary

Capitoli

00:05Titolo
03:06Set-up and Anesthesia
04:26Animal Preparation for Surgery
05:21Incision and Exposure of Uterine Horns
06:45Injection of DNA into Vesicles
08:23Electroporation
09:13Suturing and Stapling
10:12Fluorescent Protein Expression in Multiple CNS Territories of Electroporated Embryonic Mouse Brain
11:52Conclusion

Summary

Traduzione automatica

在子宫内电允许在一个空间和时间控制在发展中国家的中枢神经系统（CNS）的方式进行快速的基因传递。在这里，我们描述了一种高适应的在子宫内电的协议，可用于提供成多个胚胎中枢神经系统领域，包括端脑，间脑和视网膜的表达结构。

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