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Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis

Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis

DOI:
10.3791/2958-v

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06:42 min

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November 30, 2011

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, , ,
1Center for Cerebrovascular Research, Department of Anesthesia and Perioperative Care,University of California, San Francisco, 2Department of Neurological Surgery,University of California, San Francisco, 3Department of Neurology,University of California, San Francisco

Capitoli

  • 00:05Titolo
  • 01:30Clearing Blood from the Vascular System
  • 02:43Perfusion of Casting Agent into the Vascular System
  • 04:02Collection and Processing of Tissue for Multiple Imaging Tools
  • 04:51Representative Images of Casted Vasculature using Multiple Imaging Tools
  • 05:57Conclusion

Summary

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In this article, we present a simple, practical technique for cerebrovascular casting that is easy to perform and can be utilized to image the vascular tree of the adult mouse brain.

Tags

Cerebrovascular Casting3D ImagingMorphological AnalysisVascular ImagingCerebrovascular DiseasesBrain Arteriovenous Malformations (BAVMs)Animal ModelsVessel Imaging MethodsMurine Brain Disease ModelsMurine Cerebral Vascular TreeX-ray Catheter Contrast-based AngiographySmall RodentsCasting MaterialIntracardial PerfusionVascular CastingOrganic Solvent Methyl SalicylateThree Dimensional ImagingBrain Blood Vessels

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