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Brain Slice Stimulation Using a Microfluidic Network and Standard Perfusion Chamber
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Biologia
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JoVE Journal Biologia
Brain Slice Stimulation Using a Microfluidic Network and Standard Perfusion Chamber

Brain Slice Stimulation Using a Microfluidic Network and Standard Perfusion Chamber

DOI:
10.3791/302-v

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27:58 min

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October 01, 2007

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, , ,
1Dept. of Bioengineering,University of Illinois, Chicago, 2Department of Anatomy and Cell Biology,University of Illinois, Chicago

Capitoli

  • 00:00Titolo
  • 00:36Introduction
  • 03:33The SU8 Master
  • 04:15Replica Molding
  • 10:54Microfluidic Device Fabrication
  • 17:34Integration of Microfluidic Device with Standard Perfusion Chamber
  • 23:02Loading a Brain Slice onto the Device
  • 25:18The Utility of the Brain Slice Device for Precise Delivery of Neuro

Summary

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We demonstrate fabrication of a simple microfluidic device that can be integrated with standard electrophysiology setups to expose microscale surfaces of a brain slice in a well controlled manner to different neurotransmitters.

Tags

Brain Slice StimulationMicrofluidic NetworkStandard Perfusion ChamberElectrophysiology SetupNegative Resist LithographyMicrochannelsInlet And Outlet PortsCircular HolesPassive Pumping MethodOff-the-shelf Perfusion ChambersNeurotransmittersTransparent MaterialsMicroscopyChemical Stimuli

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