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Automatiseret hydrofobisk interaktions-kromatografi Kolonne Valg til brug i Proteinoprensning
JoVE Journal
Biologia
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JoVE Journal Biologia
Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Automatiseret hydrofobisk interaktions-kromatografi Kolonne Valg til brug i Proteinoprensning

DOI:
10.3791/3060-v

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10:21 min

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September 21, 2011

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, ,
1College of Nursing, Interdisciplinary Life Sciences Research Laboratory,Seattle University, 2College of Science and Engineering, Interdisciplinary Life Sciences Research Laboratory,Seattle University

Capitoli

  • 00:05Titolo
  • 01:30Buffer and Sample Preparation
  • 02:48Physical Setup and Plumbing of the DuoFlow Chromatography System
  • 04:11Priming System Lines,Programming Run Method, and Equilibrating HIC Columns
  • 05:43Sample Loading, Programming the Software, and Running the Column Scouting Protocol
  • 07:36Representative Chromatogram and Fluorescent Fraction Data
  • 09:23Conclusion

Summary

Traduzione automatica

Traduzione automatica

En automatiseret fremgangsmåde til identifikation af egnede hydrofobe interaktions-kromatografi (HIC) medier, der skal anvendes i processen proteinoprensning er præsenteret. Fremgangsmåden anvender en medium-tryk-væskekromatografi, der indbefatter automatiserede puffer blanding, dynamisk prøvesløjfe injektion, sekventiel kolonne, multi-bølgelængde analyse og opdelt fraktion eluat samling.

Tags

Automated Hydrophobic Interaction ChromatographyProtein PurificationChromatographic Medium SelectionScoutingHydrophobicityAffinity ChromatographyIon Exchange ChromatographyProtein ConcentrationAmmonium SulfatePurification MethodNonpolar LigandChromatography ColumnSalt Concentration

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