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Journal
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Biology
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Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs
Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs
DOI:
10.3791/308-v
•
21:38 min
•
October 01, 2007
•
Victor Chi
,
K. George Chandy
1
Department of Physiology and Biophysics
,
University of California, Irvine (UCI)
Chapters
00:02
Title
00:06
Introduction
00:49
Dewaxing and Rehydrating Paraffin Sections
03:20
Unmasking Antigen with Heat-Induced Antigen Retrieval
05:14
Blocking Endogenous Peroxidase Activity and Nonspecific Binding of
08:17
Adding Primary and Secondary Antibodies
11:39
Preparing and Incubating Slides in the ABC Reagent
13:21
Preparing and Reacting Slides with DAB Substrate Reagent
16:10
Counterstaining and Mounting the Slides
20:46
Visualizing Anti-Tyrosine Hydroxylase Slides
21:07
Conclusion
Tags
Immunohistochemistry
IHC
Paraffin Sections
Vectastain ABC Kit
Vector Labs
Protein Expression
Specific Antibodies
Direct Method
Indirect Method
Primary Antibodies
Biotin-conjugated Secondary Antibodies
Frozen Sections
Paraffin-embedded Sections
Glass Slides
Dewaxing
Hydration
Alcohol Gradient
Antigen Retrieval
Endogenous Peroxidase Activity
Non-specific Binding Sites
T Cell Marker CD8
Tyrosine Hydroxylase
Secondary Antibodies Conjugated To Biotin
Avidin-conjugated Horseradish Peroxidase (HRP)
Diaminiobenzidine (DAB)
Contrast
Coverslip
Article
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