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Biofysische Testen om de mechanische eigenschappen van de interfase celkern Probe: Basismateriaal Strain Toepassing en micronaaldjes Manipulatie
JoVE Journal
Biologia
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JoVE Journal Biologia
Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation
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Biofysische Testen om de mechanische eigenschappen van de interfase celkern Probe: Basismateriaal Strain Toepassing en micronaaldjes Manipulatie

DOI:
10.3791/3087-v

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16:27 min

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September 14, 2011

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, ,
1Brigham and Women’s Hospital / Harvard Medical School,Department of Medicine, Cardiovascular Division, 2Weill Institute for Cell and Molecular Biology & Department of Biomedical Engineering,Cornell University

Capitoli

  • 00:05Titolo
  • 01:47Preparation of Silicone Membrane Dishes and Adherence of Cells
  • 03:30Substrate Strain Experiments
  • 06:50Substrate Strain Analysis
  • 08:35Representative Results for Substrate Strain Application on the Interphase Cell Nucleus
  • 09:57Microneedle Manipulation Experiment
  • 12:19Microneedle Manipulation Analysis
  • 13:50Representative Results for Microneedle Manipulation of the Interphase Cell Nucleus
  • 15:58Conclusion

Summary

Traduzione automatica

Traduzione automatica

We presenteren twee onafhankelijke, microscoop-gebaseerde tools om de veroorzaakte nucleaire en cytoskelet vervormingen in enkele, levende hechtende cellen te meten in reactie op globale of lokale stam toepassing. Deze technieken worden gebruikt om de nucleaire stijfheid (dat wil zeggen, vervormbaarheid) te bepalen en om intracellulaire krachtoverbrenging tussen de kern en het cytoskelet sonde.

Tags

Biophysical AssaysMechanical PropertiesInterphase Cell NucleusSubstrate Strain ApplicationMicroneedle ManipulationEukaryotic CellsNucleus StiffnessCytoskeletonBiomechanical BehaviorPhysiological ConditionsPathological ConditionsMigrating NeutrophilsInvading Cancer CellsExtravasationNarrow SpacesMuscleStructural SupportRepetitive Mechanical StressCellular ArchitectureNuclear Envelope ProteinsLaminsNesprinsNucleo-cytoskeletal CouplingHuman Diseases

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