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Capitoli
- 00:05Titolo
- 01:44Establishment of Fibroblast Cultures from Skin Explants
- 02:57Stage I: Preparation of Collagen I from Rat Tails
- 04:55Stage II: Setting Up the 3D Matrix with Embedded Fibroblasts
- 06:48Stage III: Plating Cells of Interest on Top of the Matrix
- 07:33Stage IV: Transferring the Matrix to a Grid for Invasion (approximately 0-21 days)
- 08:38Stage V: Fixation
- 09:02Applications of the Organotypic Assay
- 10:18Conclusion
Tags
Organotypic Collagen I AssayCell Behavior3-dimensional ContextCell MigrationBiologiaDevelopmentWound HealingImmune SystemTumor CellsGlass CoverslipsLight MicroscopyLocal EnvironmentElasticityProtein CompositionPore SizeTwo-dimensional SubstratesStromal FibroblastsImmune CellsInvasion Of Cancer CellsMolecular DynamicsDrug Treatmentresponse To Drug TreatmentIn Vitro And In Vivo ComparisonCell Derived MatrixMatrigelOrganotypic Culture
