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选择恶性疟原虫寄生虫对人脑血管内皮细胞
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Selection of Plasmodium falciparum Parasites for Cytoadhesion to Human Brain Endothelial Cells
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选择恶性疟原虫寄生虫对人脑血管内皮细胞

DOI:
10.3791/3122-v

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10:09 min

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January 03, 2012

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,
1Centre for Immunity, Infection and Evolution,University of Edinburgh

Capitoli

  • 00:05Titolo
  • 01:14Preparation and Routine Culture of Plasmodium falciparum
  • 02:54Routine Culture of Endothelial Cells and Preparation for Selection
  • 04:00Selection of P. falciparum for Cytoadhesion to Endothelial Cells
  • 07:36Increased Binding of Parasites to HBEC-5i Cells after Selection
  • 09:43Conclusion

Summary

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一个<em>在体外</em>脑型疟疾封存模型描述<sup> 1</sup>。<em>恶性疟原虫</em>受感染的红细胞被选中人脑微血管内皮细胞结合。选定的寄生虫表现出不同的表型。选择过程中可以使用各种应用<em> P。恶性疟原虫</em>株和血管内皮细胞株。

Tags

Plasmodium FalciparumCytoadhesionHuman Brain Endothelial CellsCerebral MalariaMicrovasculatureSequestrationBlood Vessel WallsHBEC-5iIn Vitro ModelPanning AssayHigh-binding ParasitesPigmented Trophozoite Stage

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