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1Temple University,Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology, 2Medical Research Service,Department of Veterans Affairs Hospital, 3Department of Neurobiology and Anatomy,Drexel University College of Medicine, 4Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology,Temple University School of Medicine
Capitoli
- 00:05Titolo
- 01:19Preparation for Surgery
- 02:19Laminectomy and Surgical Exposure of the L5 Dorsal Root
- 03:21Rhizotomy/Dorsal Root Crush
- 04:10Image Acquisition and Post-op Procedures
- 04:51Repeated Imaging
- 05:32Axonal Regeneration Following Dorsal Root Crush
- 06:16Conclusion
एक<em> Vivo में</em> इमेजिंग पृष्ठीय रूट कुचलने के बाद प्राथमिक संवेदी axons की निगरानी प्रोटोकॉल में वर्णित है. प्रक्रियाओं के व्यापक क्षेत्र प्रतिदीप्ति माइक्रोस्कोपी और thy1 YFP ट्रांसजेनिक चूहों का उपयोग, और अक्षतंतु उत्थान के पीएन और अक्षतंतु सीएनएस के अंतरफलक के साथ बातचीत में 4 सेमी से अधिक दोहराया इमेजिंग परमिट.
Tags
Live ImagingDorsal Root AxonsRhizotomySpinal Root InjuriesChronic PainSensory LossRegenerationDorsal Root Entry Zone (DREZ)Molecular EventsCellular EventsNerve InjuryPostmortem AnalysesAxon RegenerationReal-time EventsLiving AnimalNeurons Serially In VivoOpticsMouse TransgenicsThy1-GFP Transgenic MiceFluorescent ColorsIn Vivo ImagingMuscleBrainPhysiological MechanismsStatic AnalysesSpinal Cord Imaging
