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一个用于标识Allelotyping PCR扩增沙门氏菌肠炎血清型，哈达尔，海德堡和鼠伤寒沙门氏菌
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
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一个用于标识Allelotyping PCR扩增沙门氏菌肠炎血清型，哈达尔，海德堡和鼠伤寒沙门氏菌

DOI:
10.3791/3130-v

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12:18 min

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July 22, 2011

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, , ,
1Department of Population Health,University of Georgia

Capitoli

  • 00:05Titolo
  • 02:00Preparation of Whole Cell Template
  • 03:29PCR Set-up
  • 06:45Gel Electrophoresis
  • 09:20Multiplexing PCR Results: Identifying Specific O and H Alleles Associated with S. enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
  • 11:51Conclusion

Summary

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我们描述了一个多重PCR快速检测沙门氏菌血清型肠炎，哈达尔，海德堡和鼠伤寒沙门氏菌。具体的沙门氏菌血清型，可确定的目标是多重PCR技术，以独特的O -抗原生物合成集群和一个给定的血清型的鞭毛的基因和序列。血清型，然后分配到一个隔离基础上的具体化，规模扩增产物（PCR产物）对应的目标等位基因外观的沙门氏菌。

Tags

Allelotyping PCRIdentifying Salmonella Enterica SerovarsEnteritidisHadarHeidelbergTyphimuriumCommercial PCR TestsSalmonella Target GenesSalmonella SerovarsPublic Health SignificanceS. Enterica Subspecies IIIa ArizonaS. Enterica Subspecies I Serovar EnteritidisSalmonellosisTable EggsLipopolysaccharide (LPS)Flagellin (H1 And H2 Antigens)Antigenic DifferencesGenetic DifferencesAllelotyping Multiplex PCRPoultry-associated DiseasesUS

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