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Profiling Voltage-gated Potassium Channel-mRNA Expression in nigralen Neuronen mit Einzel-Zell RT-PCR-Techniken
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Capitoli
- 00:05Titolo
- 00:54Brain Slice Preparation
- 01:42Electrophysiological Fingerprinting of Nigral Neurons
- 02:48Cytoplasm Aspiration
- 03:29Reverse Transcription (RT) Reaction
- 04:23Two Stage PCR Amplification
- 05:14Electrophysiology and Gene Expression in Selected Neurons
- 07:04Conclusion
Neuronen sind zunächst geprägt elektrophysiologisch. Dann das Zytoplasma aus den aufgezeichneten Neuronen wird abgesaugt und einer Transkription-PCR-Analyse umzukehren, um die Expression von mRNAs für Neurotransmitter-Synthese-Enzyme, Ionenkanäle und Rezeptoren zu erkennen.
Tags
ProfilingVoltage-gated Potassium ChannelMRNA ExpressionNigral NeuronsSingle-cell RT-PCR TechniquesMammalian Central Nervous SystemNeuronsMolecular CharacteristicsFunctional CharacteristicsMorphologyGene Expression AnalysisSpecific Neuron TypeWhole-cell Patch Clamp Recording TechniquesSingle-cell Reverse Transcription Polymerase Chain Reaction (scRT-PCR)MRNA Expression ProfilingSubstantial NigraElectrophysiological TechniquesNeurophysiological PropertiesFunctional PropertiesCytoplasm AspirationScRT-PCR AnalysisNeurotransmitter Synthesis EnzymesReceptorsIon ChannelsSelectivitySensitivityImmunohistochemistryAntibody SuitabilityProtein Expression Level
