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增加从单细胞的数量标准实验室逆转录反应的mRNA的cDNA产量，使用声Microstreaming
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JoVE Journal Bioingegneria
Increasing cDNA Yields from Single-cell Quantities of mRNA in Standard Laboratory Reverse Transcriptase Reactions using Acoustic Microstreaming
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增加从单细胞的数量标准实验室逆转录反应的mRNA的cDNA产量，使用声Microstreaming

DOI:
10.3791/3144-v

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04:52 min

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July 11, 2011

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, , , , ,
1Florey Neuroscience Institutes and Centre for Neuroscience,University of Melbourne, 2Fluid Dynamics Group,CSIRO Materials Science and Engineering, 3Swinburne University of Technology,Faculty of Engineering and Industrial Sciences

Capitoli

  • 00:05Titolo
  • 01:43Micromixing an RT Reaction
  • 02:59The Micromixing Advantage in RT
  • 04:10Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们描述了一个从单细胞的mRNA数量增加在其他标准的实验室逆转录反应的cDNA产量的新方法。求新驻留在一个微混合器的使用，它采用的声学microstreaming现象，微升尺度混合流体比摇晃，震荡或trituration更有效。

Tags

CDNA YieldsSingle-cell QuantitiesMRNALaboratory Reverse Transcriptase ReactionsAcoustic MicrostreamingGene ExpressionSingle-cell LevelLabile MRNADegradationStable CDNA CopyEfficiencyStandard ReagentsMicroliter VolumesMixing ReagentsMicro-scale Mixing TechniquesMicrofluidic-based Mixing TechnologiesRT Reaction Yields

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