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Cell-based Calcium Assay for Medium to High Throughput Screening of TRP Channel Functions using FlexStation 3
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Capitoli
- 00:05Titolo
- 01:41Cell Preparation
- 02:52Calcium Assay Preparation
- 04:37Plate Reading in FlexStation 3 and Data Analysis
- 05:48Representative Calcium Assay Results
- 07:05Conclusion
Tags
Cell-based Calcium AssayMedium To High Throughput ScreeningTRP Channel FunctionsFlexStation 3Multi-mode Microplate ReaderFluorescence MeasurementMulti-well PlatesAcademic SettingsIntegrated Fluid Transfer ModuleMulti-channel PipetterFluorescence ChangesCa2+-permeable Ion ChannelsG-protein Coupled ReceptorsIntracellular Free Ca2+ LevelsTransient Receptor Potential (TRP) ChannelsCalcium InfluxTRPA1 ChannelMolecular SensorNoxious StimuliHEK293 CellsCa2+-sensitive Fluorescent DyeFluo-4Real-time Fluorescence ChangesTRPA1 AgonistTRPA1 Antagonist
