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对赫斯特的分离与表征^低 CD45^负小鼠肺间质干细胞

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Isolation & Characterization of Hoechst^low CD45^negative Mouse Lung Mesenchymal Stem Cells

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对赫斯特的分离与表征^低 CD45^负小鼠肺间质干细胞

DOI:

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16:55 min

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October 26, 2011

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Kelsey S. Chow², DuHyun Jun², Karen M. Helm, David H. Wagner^2,4, Susan M. Majka^2,3

¹Charles C. Gates Regenerative Medicine and Stem Cell Biology Program,University of Colorado Denver, ²Department of Medicine,University of Colorado Denver, ³Cancer Center,University of Colorado Denver, ⁴Webb Waring Institute,University of Colorado Denver

Capitoli

00:05Titolo
01:49Lung Isolation
04:05Preparation of a Single Cell Suspension from Lung Tissue
07:15Flow Cytometry Analysis to Define and Isolate a Lung MSC Population using Hoechst dye Efflux to Detect a Side Population
13:16Enumeration of Lung MSC using a Colony Forming Assay (CFU-F)
14:49Representative Analysis of Isolated MSCs
16:08Conclusion

Summary

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在这篇文章中，我们展示了居民小鼠肺间质干细胞（肺海安），他们的扩张，表征和分析免疫属性的隔离。

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