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Multiparametric Optical Mapping of the Langendorff-perfused Rabbit Heart
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Multiparametric Optical Mapping of the Langendorff-perfused Rabbit Heart

Multiparametric Optical Mapping of the Langendorff-perfused Rabbit Heart

DOI:
10.3791/3160-v

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09:24 min

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September 13, 2011

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, ,
1Department of Biomedical Engineering,Washington University in St. Louis

Capitoli

  • 00:05Titolo
  • 02:29Harvesting and Perfusion of Rabbit Hearts
  • 03:33Conducting Experiments Using the Panoramic Optical Mapping System
  • 05:39Conducting Experiments Using the Dual Mapping System
  • 07:29Representative Results
  • 08:48Conclusion

Summary

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This article describes the basic procedures for conducting optical mapping experiments in the Langendorff-perfused rabbit heart using the panoramic imaging system, and the dual (voltage and calcium) imaging modality.

Tags

Multiparametric Optical MappingLangendorff-perfused Rabbit HeartOptical ImagingFluorescent ProbesCardiac ElectrophysiologyCalcium-sensitive DyesVoltage-sensitive DyesTransmembrane Action PotentialsCalcium TransientsSpatial ResolutionElectrode TechniquesLangendorff-perfused Rabbit Heart ModelArrhythmiasWave PatternsVentricular ArrhythmiasExcitation-contraction CouplingRabbit Myocardium

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