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Mouse Eye Enucleation for Remote High-throughput Phenotyping

JoVE Journal
Medicina
Author Produced

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JoVE Journal Medicina

Mouse Eye Enucleation for Remote High-throughput Phenotyping

Mouse Eye Enucleation for Remote High-throughput Phenotyping

DOI:

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05:30 min

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November 19, 2011

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Vinit B. Mahajan², Jessica M. Skeie², Amir H. Assefnia³, MaryAnn Mahajan², Stephen H. Tsang⁴

¹Department of Ophthalmology and Visual Sciences,University of Iowa, ²Omics Laboratory,University of Iowa, ³School of Dentistry,UCLA, ⁴Bernard and Shirlee Brown Glaucoma Laboratory, Department of Ophthalmology, College of Physicians and Surgeons,Columbia University

Capitoli

00:05Titolo
01:20Blunt Enucleation
02:27Posterior Limbal Puncture
03:00Transfer to Fixative
03:14Sharp Dissection
04:38Transport and Shipping

Summary

Traduzione automatica

The dissection technique illustrates enucleation of the mouse eye for tissue fixation to perform phenotyping in high-throughput screens.

Tags

Mouse Eye Enucleation Remote High-throughput Phenotyping Genetic Model Ophthalmic Disease Macular Degeneration Photoreceptor Degeneration Cataract Glaucoma Retinoblastoma Diabetic Retinopathy Transgenic Mice Genotype-phenotype Correlations Ophthalmic Phenotyping Surgical Skill Transfer Molecular Phenotyping Morphological Phenotyping

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