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1Diabetes and Obesity Research Center,Sanford-Burnham Medical Research Institute at Lake Nona, 2Department of Molecular Physiology and Biophysics,Vanderbilt University School of Medicine, 3Vanderbilt Mouse Metabolic Phenotyping Center,Vanderbilt University School of Medicine, 4Department of Pediatrics and Cellular and Integrative Physiology,Indiana University School of Medicine
Capitoli
- 00:05Titolo
- 01:23Surgery
- 05:21Hyperinsulinemic-euglycemic Clamp
- 09:09Evaluation of Insulin Action in Chow-fed vs. High Fat-fed Obese Mice using the Hyperinsulinemic-euglycemic Clamp
- 10:34Conclusion
Il iperinsulinemico-euglicemico morsetto, o pinza insulina, è il gold standard per valutare l'azione dell'insulina<em> In vivo</em>. Un metodo per l'esecuzione di fascette di insulina nei topi è descritto. Ciò include un metodo per cateterismo arterioso che permette esperimenti da eseguire in coscienza, i topi sfrenato con un minimo sforzo.
Tags
Hyperinsulinemic-euglycemic ClampsInsulin ActionType 2 DiabetesInsulin InfusionGlucose InfusionGlucose Infusion Rate (GIR)Whole-body Insulin ActionIsotopic 2[14C]deoxyglucose[3-3H]glucoseEndogenous Glucose Appearance (endoRa)Hepatic Glucose ProductionWhole-body Glucose Disappearance (Rd)Genetic Mouse Models Of Metabolic DiseaseDiabetes ResearchInsulin Clamp Performance
