Rivista
/
Biologia
/
Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay
JoVE Journal
Biologia
Author Produced
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay

Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay

DOI:
10.3791/3202-v

•

10:28 min

•

January 08, 2012

•

, ,
1Obstetrics, Gynecology and Women’s Health,University of Missouri

Capitoli

  • 00:05Titolo
  • 01:30Dissection of the Mouse Placenta
  • 03:29Isolation of Trophoblast Cells
  • 06:53Trophoblast Invasion Assay
  • 08:21Representative Results

Summary

Traduzione automatica

Traduzione automatica

In this protocol, we describe the dissection of placentae from the mouse on pregnancy d10.5, followed by isolation of trophoblast cells using a Percoll gradient. We then demonstrate use of the isolated cells in a matrigel invasion assay.

Tags

IsolationPrimary Mouse Trophoblast CellsTrophoblast Invasion AssayPlacentaNutrientsGassesGrowth FactorsWastesPlacental DevelopmentFetusMotherEmbryonic LethalityCell TypeFetal PortionToolNormal Placental DevelopmentAbnormal Placental DevelopmentCell LinesSpecific Stages Of PregnancyPrimary CulturesTransgenic MiceParticular GenesPercoll GradientTrophoblast Cell PopulationMouse PlacentasHuman Trophoblast Cell IsolationPurity AssessmentImmunocytochemical StainingCytokeratin 74Matrigel Invasion AssayTrophoblast Invasiveness In Vitro

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/3202?article_type=v

Derivation of Mouse Trophoblast Stem Cells from Blastocysts
check_url/it/3202?article_type=v

Isolation and Primary Culture of Rat Hepatic Cells
check_url/it/3202?article_type=v

Isolation of Immune Cells from Primary Tumors
check_url/it/3202?article_type=v

Isolation and Culture of Mouse Primary Pancreatic Acinar Cells
check_url/it/3202?article_type=v

Robust 3D DNA FISH Using Directly Labeled Probes
check_url/it/3202?article_type=v

Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue
check_url/it/3202?article_type=v

Isolation and Primary Culture of Mouse Aortic Endothelial Cells
check_url/it/3202?article_type=v

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
check_url/it/3202?article_type=v

Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells
check_url/it/3202?article_type=v

Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs

Read Article