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高活性酵母核糖体的色谱分离纯化
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Biologia
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JoVE Journal Biologia
Chromatographic Purification of Highly Active Yeast Ribosomes
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高活性酵母核糖体的色谱分离纯化

DOI:
10.3791/3214-v

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09:13 min

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October 24, 2011

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, ,
1Department of Cell Biology and Molecular Genetics,University of Maryland , 2Department of Biotechnology and Microbiology,Vilnius University

Capitoli

  • 00:05Titolo
  • 01:17Preparation of a Cysteine Charged Sulfolink Resin
  • 02:36Chromatographic Purification of Ribosomes Using Cysteine Charged Sulfolink Resin
  • 05:18Puromycin Treatment & Purification of Ribosomes by Sedimentation Through Glycerol Cushions
  • 07:28Representative Purification of Highly Active Yeast Ribosomes
  • 08:48Conclusion

Summary

Traduzione automatica

Traduzione automatica

准备用传统的方法，纯化共同净化核酸酶和蛋白酶的真核细胞的核糖体的污染造成了消极影响下游的生化和结构分析。一个快速和简单的色谱的净化方法是用来解决这个问题作为一个模型系统中使用的酵母核糖体。

Tags

Chromatographic PurificationYeast RibosomesLabile RibosomesProteasesNucleasesDifferential UltracentrifugationStructural IntegrityFunctionalityCysteine Charged Sulfolink ResinCo-purifying Proteolytic And Nucleolytic ActivitiesIntact RibosomesBiochemically Active RibosomesMammalian RibosomesTechnical Advancement

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