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Anvendelse af en mus Ligated Peyer er Patch Intestinal Loop Assay at evaluere Bakteriel Optagelse ved M celler
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells
DOI:

05:59 min

December 17, 2011

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Capitoli

  • 00:05Titolo
  • 01:12Preparation of Bacterial Cells
  • 02:26Ligated Peyer’s Patch Loop Assay
  • 03:42Whole Mount Staining and Confocal Microscopic Analysis of Peyer’s Patch
  • 05:12Localization of Fluorescent S. Typhimurium and GP2 in M Cells
  • 05:36Conclusion

Summary

Traduzione automatica

M celler i en specialiseret follikel-associerede epitel dækker Peyerske plaques spiller en vigtig rolle for mucosal immunosurveillance i tarmen-associerede lymfoide væv. Her har vi beskrev evalueringsmetode for bakteriel transcytosis af M celler<em> In vivo</em>. Denne metode giver en metode til at forstå M-celle funktion i immunsystemet.

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