Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical Footprinting

Ravichandra Bachu; Frances-Camille S. Padlan; Sara Rouhanifard; Michael Brenowitz; J&#246;rg C. Schlatterer

doi:10.3791/3244

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Biologia

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监测RNA结构平衡的变动情况“过氧化”和“氧化”羟基自由基足

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Biologia

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JoVE Journal Biologia

Monitoring Equilibrium Changes in RNA Structure by ‘Peroxidative’ and ‘Oxidative’ Hydroxyl Radical Footprinting

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监测RNA结构平衡的变动情况“过氧化”和“氧化”羟基自由基足

DOI:

10.3791/3244-v

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13:41 min

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October 17, 2011

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Ravichandra Bachu*¹, Frances-Camille S. Padlan*², Sara Rouhanifard, Michael Brenowitz, Jörg C. Schlatterer

¹Department of Chemistry,Hunter College , ²Department of Biochemistry,Albert Einstein College of Medicine

Capitoli

00:05Titolo
02:24RNA Preparation for Footprinting Experiment
04:24The Footprinting Experiment
08:09Data Analysis
10:38Analysis of RNA Fragments after Peroxidative Fenton Footprinting Reaction
12:23Conclusion

Summary

Traduzione automatica

本协议描述了如何量化的镁（二）依赖RNA的三级结构的形成由两个羟基自由基足迹方法。

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