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Optical Mapping of Action Potentials and Calcium Transients in the Mouse Heart
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Bioingegneria
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JoVE Journal Bioingegneria
Optical Mapping of Action Potentials and Calcium Transients in the Mouse Heart

Optical Mapping of Action Potentials and Calcium Transients in the Mouse Heart

DOI:
10.3791/3275-v

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08:13 min

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September 13, 2011

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, , ,
1Department of Biomedical Engineering,Washington University in St. Louis

Capitoli

  • 00:05Titolo
  • 01:24Preparing the Perfusion and Imaging Setup
  • 03:23Langendorff Perfusion of the Isolated Heart
  • 05:25Loading Voltage and Calcium Sensitive Dyes
  • 06:38Optical Imaging and Data Analysis
  • 07:47Conclusion

Summary

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This paper details the dissection procedure, instrumental setup, and experimental conditions during optical mapping of transmembrane potential (Vm) and intracellular calcium transient (CaT) in intact isolated Langendorff perfused mouse hearts.

Tags

Optical MappingAction PotentialsCalcium TransientsMouse HeartCardiovascular StudiesFluorescence ImagingGenetic EngineeringPhysiological PhenotypingTransmembrane Potential (Vm)Excitation-contraction CouplingLangendorff Perfused Mouse HeartsHeart FailureArrhythmiasMetabolic DiseaseHeart DiseasesActivation VisualizationConduction VelocityAction Potential DurationInstrumentation SetupExperimental ConditionsSpatio-temporal ResolutionCMOS Imaging Technology

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