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Single Cell transkriptionel Profilering af Voksen Mouse cardiomyocytter
JoVE Journal
Biologia
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JoVE Journal Biologia
Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Single Cell transkriptionel Profilering af Voksen Mouse cardiomyocytter

DOI:
10.3791/3302-v

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08:23 min

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December 28, 2011

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, ,
1Buck Institute for Research on Aging, 2Department of Physiology & Biophysics,University of Washington

Capitoli

  • 00:05Titolo
  • 01:15Isolating Cardiomyocyte
  • 03:37Isolating Single Cells
  • 04:08Single Cell qPCR
  • 05:26Whole Transcriptome Amplification and Microarray of Single Cells
  • 07:00Quality Control Results
  • 07:56Conclusion

Summary

Traduzione automatica

Traduzione automatica

Enkelt celle udtryk profilering giver den detaljerede genekspression analyse af de enkelte celler. Vi beskriver metoder til isolering af cardiomyocytter, og forberede den resulterende lysates for enten hele transkriptom microarray eller qPCR af specifikke mål.

Tags

Single CellTranscriptional ProfilingAdult MouseCardiomyocytesGene ExpressionHomogenatesStochastic VariationPure Cardiomyocyte PopulationsCollagenase PerfusionMicroarraysWhole TranscriptomeQPCRNanofluidic ArraysGene Expression ProfilesEvaluation MetricsVariance Between CellsRobust AmplificationDouble Stranded CDNANimbleGen ArraysReverse Transcriptase – Specific Target Amplification (RT-STA)Nanofluidic PCRVariability Of Expression

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