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Capitoli
- 00:05Titolo
- 01:36Initiating the ZFN Design Process
- 03:23Custom ZFN Production and Validation
- 04:16Delivery of Validated ZFNs by Nucleofection
- 06:25Cel-1 Assay
- 07:09Clonal Isolation and Characterization
- 07:46Estimated ZFN Activity from the Cel-1 Assay
- 08:27Conclusion
Den CompoZr tilpassede zink-finger-nuklease (ZFN) Service muliggør præcis genom redigering i en organisme eller celle linie ved ethvert sted defineret af brugeren. Denne artikel beskriver processen for konstruktion, fremstilling, validering og implementering af CompoZr Brugerdefineret ZFN Service.
Tags
Genome EditingCompoZr Custom Zinc Finger NucleasesGene FunctionGenetic Basis Of DiseaseTraditional Gene Editing MethodsChemical-based MutagenesisRandom Integration Of DNA SequencesSynthetic SequencesAberrant Culture ConditionsTransient ZFN ExpressionPrecise Gene EditingHighly Efficient MannerZinc Finger NucleasesDouble-stranded DNA (dsDNA)CompoZr ZFN UnitDNA-cleavage DomainsDouble-strand Break (DSB)Non-homologous End-joining (NHEJ) DNA Repair PathwaySmall Insertions And Deletions (indels)FrameshiftFunctional Knockout
