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构建一个低预算的激光干切断系统研究轴突再生 C。线虫</em
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
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构建一个低预算的激光干切断系统研究轴突再生 C。线虫</em

DOI:
10.3791/3331-v

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10:05 min

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November 15, 2011

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, ,
1Department of Biology,University of Utah

Capitoli

  • 00:05Titolo
  • 01:39Construction of a Laser Ablation System
  • 03:42Aligning the Laser Beam
  • 04:54Expanding and Focusing the Laser Beam
  • 08:29Axotomies using the Assembled System
  • 09:21Conclusion

Summary

Traduzione automatica

Traduzione automatica

激光干切断时间推移成像是一个敏感的方法检测基因突变的影响<em> C。线虫</em>轴突再生。一支素质高，但价格低廉，激光消融系统可以很容易地添加到最显微镜。超过15小时的时间的推移成像需要小心固定的蠕虫病毒。

Tags

Low-budgetLaser Axotomy SystemStudyAxon RegenerationC. ElegansAssayCostTechnical ExpertiseLaser Ablation SystemSolid-state Pulse LasersTransparent PreparationsConstructionAlignmentOptical PathFocused CondenserIntermediate ModuleDichroicImagingLaser BeamMicroscope Condenser LensObjective LensFinal AlignmentTesting

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