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La construcción de un láser de bajo presupuesto axotomía Sistema para el Estudio de la regeneración de axones en C. elegans</em
JoVE Journal
Bioingegneria
This content is Free Access.
JoVE Journal Bioingegneria
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
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10:05 min

November 15, 2011

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Capitoli

  • 00:05Titolo
  • 01:39Construction of a Laser Ablation System
  • 03:42Aligning the Laser Beam
  • 04:54Expanding and Focusing the Laser Beam
  • 08:29Axotomies using the Assembled System
  • 09:21Conclusion

Summary

Traduzione automatica

Laser axotomy followed by time-lapse imaging is a sensitive way to assay the effects of mutations in C. elegans on axon regeneration. A high quality, but inexpensive, laser ablation system can be easily added to most microscopes. Time lapse imaging over 15 hours requires careful immobilization of the worm.

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