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Ortogonale Proteinoprensning Faciliteret af en lille Bispecific Affinity Tag
JoVE Journal
Biologia
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JoVE Journal Biologia
Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

Ortogonale Proteinoprensning Faciliteret af en lille Bispecific Affinity Tag

DOI:
10.3791/3370-v

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10:32 min

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January 16, 2012

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, ,
1School of Biotechnology, Department of Proteomics,Royal Institute of Technology

Capitoli

  • 00:05Titolo
  • 02:05Cloning of the Target Gene/ABDz1-tagged Fusion Construct
  • 04:02Protein Expression
  • 04:49Orthogonal Affinity Purification
  • 08:04Results: SDS-PAGE Analysis of Purified Protein Products
  • 09:45Conclusion

Summary

Traduzione automatica

Traduzione automatica

En ny og meget effektiv to-trins affinitetskromatografi protokol, er blevet udviklet og er beskrevet i detaljer. Metoden er baseret på et lille rensning tag med to iboende tilhørsforhold, og kan anvendes på en bred vifte af target-proteiner med forskellige egenskaber.

Tags

Orthogonal Protein PurificationSmall Bispecific Affinity TagRecombinant ProteinsPurification ProtocolsHigh-throughput MethodsPurification TagsHexa-histidine TagAffinity ChromatographyBispecific Purification TagAlbumin-binding DomainStreptococcal Protein GHuman Serum Albumin (HSA)Phage Display TechnologyDimeric Z-domainStaphylococcal Protein A

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