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2Dと3D環境での神経前駆細胞の電界制御監督移行
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Electric Field-controlled Directed Migration of Neural Progenitor Cells in 2D and 3D Environments
DOI:

11:15 min

February 16, 2012

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Capitoli

  • 00:05Titolo
  • 01:39Preparation of the Electrostatic Chamber
  • 02:54Neural Progenitor Cell Isolation
  • 04:09Application of the Electric Field to the Electrostatic Chamber
  • 07:18Preparation of the Organotypic Spinal Cord Slice
  • 08:40Injection of Hoechst 33342 Labeled Neural Progenitor Cells into the Organotypic Spinal Cord Slice
  • 09:38Results: Neural Progenitor Cells Show Directed Migration in the Electric Field and the Organotypic Spinal Cord Slice
  • 10:46Conclusion

Summary

Traduzione automatica

このプロトコルは、細胞を追跡することができますカスタム設計electrotactic室で2Dと3D環境を確立するために使用されるメソッドを示しています<em>生体/ ex vivoでの</em>直流(DC)電界(EFS)を指示する走電性および他の細胞応答を調べるために、単一細胞レベルでの経時的記録を使用します。

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