Rivista
/
Biologia
/
Growth Assays to Assess Polyglutamine Toxicity in Yeast
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Growth Assays to Assess Polyglutamine Toxicity in Yeast

Growth Assays to Assess Polyglutamine Toxicity in Yeast

DOI:
10.3791/3461-v

•

09:06 min

•

March 05, 2012

•

1Boston Biomedical Research Institute

Capitoli

  • 00:05Titolo
  • 01:25Experimental Considerations and Set-up of Yeast Cells Expressing Toxic PolyQ-expansion Proteins
  • 03:26Plating Assay
  • 04:30Spotting Assay
  • 05:25Liquid Growth Assay
  • 07:08Representative Growth Assay Results
  • 08:30Conclusion

Summary

Traduzione automatica

Traduzione automatica

This manuscript describes three complementary protocols for assessing the toxicity of polyglutamine (polyQ)-expansion proteins in the yeast Saccharomyces cerevisiae. These protocols can easily be modified to monitor the toxicity of other misfolded proteins in yeast.

Tags

Growth AssaysAssessPolyglutamine ToxicityYeastProtein MisfoldingNeurodegenerative DiseasesHuntington’s DiseaseCellular ToxicityPolyQ-expansionsSpinocerebellar AtaxiasKennedy’s DiseaseModel Organism YeastCellular PathwaysExperimental SystemsHD PatientsBasic MechanismsGrowth Defects

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/3461?article_type=v

Identification of Growth Inhibition Phenotypes Induced by Expression of Bacterial Type III Effectors in Yeast
check_url/it/3461?article_type=v

Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin
check_url/it/3461?article_type=v

Long-term Lethal Toxicity Test with the Crustacean Artemia franciscana
check_url/it/3461?article_type=v

Methods to Assess Subcellular Compartments of Muscle in C. elegans
check_url/it/3461?article_type=v

Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays
check_url/it/3461?article_type=v

Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products
check_url/it/3461?article_type=v

ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth
check_url/it/3461?article_type=v

Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast
check_url/it/3461?article_type=v

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm
check_url/it/3461?article_type=v

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Read Article