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RNAi-medieret Gene Knockdown og<em> In Vivo</em> Diurese Assay i Voksen Kvinde<em> Aedes aegypti</em> Myg
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Biologia
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JoVE Journal Biologia
RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes

RNAi-medieret Gene Knockdown og<em> In Vivo</em> Diurese Assay i Voksen Kvinde<em> Aedes aegypti</em> Myg

DOI:
10.3791/3479-v

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08:47 min

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July 14, 2012

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, , ,
1Biology Department,New Mexico State University, 2Molecular Biology Department,New Mexico State University

Capitoli

  • 00:05Titolo
  • 01:48Injection Preparation
  • 03:07Mosquito Injection
  • 04:26Mosquito Recovery and Storage
  • 04:53In vivo Diuresis Assay
  • 05:32Injection Preparation
  • 06:16Mosquito Injection
  • 06:41Weighing Mosquitoes
  • 07:27Risultati
  • 07:51Conclusions

Summary

Traduzione automatica

Traduzione automatica

I denne protokol vi kombinerer RNAi-medieret gen silencing med en<em> In vivo</em> Diurese assay til at studere virkningerne knockdown af gener af interesse har på myg fluid udskillelse.

Tags

RNAi-mediated Gene KnockdownIn Vivo Diuresis AssayAedes Aegypti MosquitoesGene Knockdown TechniqueBioassayDiuresis In InsectsAquaporin GenesMosquito Injection DeviceDsRNA InjectionExcretion Rates

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