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Propagating and Detecting an Infectious Molecular Clone of Maedi-visna Virus that Expresses Green Fluorescent Protein
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Propagating and Detecting an Infectious Molecular Clone of Maedi-visna Virus that Expresses Green Fluorescent Protein

Propagating and Detecting an Infectious Molecular Clone of Maedi-visna Virus that Expresses Green Fluorescent Protein

DOI:
10.3791/3483-v

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08:49 min

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October 09, 2011

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,
1Institute for Experimental Pathology,University of Iceland

Capitoli

  • 00:05Titolo
  • 01:19Transfection
  • 05:13Titration of Virus
  • 07:13FACS Analysis of Infected Cells

Summary

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We describe a molecular clone of maedi-visna virus that expresses GFP and is fully infectious. Replication of this virus can be detected by using fluorescence microscopy and flow cytometry.

Tags

Maedi-visna VirusLentivirusInterstitial PneumoniaEncephalitisTarget CellsMonocyte LineageCell TypesGreen Fluorescent ProteinEgfp GeneDUTPase GeneReplicationCAEVMolecular CloneIn VitroIn VivoPrimary Cell LineChoroid PlexusTransfectionPropagationInfectiousEGFP ExpressionPassagesTCID50Infected CellsCell TropismPathogenicity

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