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Intravital Imaging af Mouse Thymus med 2-Photon Microscopy
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy

Intravital Imaging af Mouse Thymus med 2-Photon Microscopy

DOI:
10.3791/3504-v

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10:08 min

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January 07, 2012

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, ,
1Laboratory of Immune Regulation,Instituto Gulbenkian de Ciência

Capitoli

  • 00:01Titolo
  • 00:05Introduction
  • 01:15Animal preparation: Irradiation and bone marrow transfer
  • 03:16Animal preparation: Thymic transplantation
  • 04:58Animal preparation: Surgery to exposure transplanted thymus
  • 06:53Two-photon image acquisition
  • 08:35Risultati

Summary

Traduzione automatica

Traduzione automatica

Vi har udviklet nye laboratorie-værktøjer og protokoller for intravital billeddannelse erhvervelse af brissel. Vores teknik skal hjælpe med identifikationen af ​​"nicher" i thymus, hvor T-celle udvikling sker.

Tags

Intravital ImagingMouse Thymus2-photon MicroscopyImage AcquisitionOrgansStereotactic ToolsSurgical ProceduresCellular BehaviorsLive AnimalsEx Vivo Imaging AcquisitionSurgical ProcedureOrgan HolderFoxp3+ CellsRegulatory T CellsNaturally-occurring Tregs (nTregs)Peripherally-converted Tregs (pTregs)In Vivo InteractionsPhenotype And PhysiologyNude Mice

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