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血管钙的可视化 2 +信号旁分泌源性活性氧引发
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Biologia
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JoVE Journal Biologia
Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS
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血管钙的可视化 2 +信号旁分泌源性活性氧引发

DOI:
10.3791/3511-v

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07:33 min

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December 21, 2011

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, , ,
1Department of Biochemistry,Temple University , 2Department of Anesthesiology and Pain Medicine,University of Washington

Capitoli

  • 00:05Titolo
  • 01:18Oxidation-sensitive Dyes: Visualization of ROS in J774 Cells
  • 02:24Fluorescent Sensor HyPer: Visualization of ROS in Stably Transfected MPMVECs
  • 04:04Co-Culture Model for Paracrine-Derived ROS
  • 05:13ROS Dynamics
  • 06:50Conclusion

Summary

Traduzione automatica

Traduzione automatica

一种有效的方法获得可视化的旁分泌衍生活性氧诱导内皮细胞Ca2 +信号的见解的描述。这种方法测量旁分泌衍生活性氧引发共培养模型在血管内皮细胞内Ca2 +动员的优势。

Tags

Vascular Ca2+ SignalingParacrineROS (reactive Oxygen Species)Oxidative StressIschemia/reperfusion DamageSepsisSignal TransductionCell ProliferationCell DeathInflammatory CellsCytokinesTumor Necrosis Factor-alpha (TNF-alpha)IL-1Endothelial Cells (EC)Endothelial DysfunctionMacrophagesNADPH Oxidases

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