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重组细小病毒与腺病毒派生系统的帮助下高效生产
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems
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重组细小病毒与腺病毒派生系统的帮助下高效生产

DOI:
10.3791/3518-v

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13:47 min

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April 23, 2012

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, , , ,
1Tumour Virology Division F010,German Cancer Research Center (DKFZ), 2Inserm Unit 701,German Cancer Research Center (DKFZ)

Capitoli

  • 00:05Titolo
  • 03:28Production of recPVs via Transfection
  • 07:09Production of recPVs via Infection
  • 11:00Results: Improved recPV Production by Adenovirus Genomic Elements
  • 13:17Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们描述只对细胞的感染，从而提高重组细小的生产效率超过100倍相比，在使用中的其他协议为基础的协议。此协议依赖于使用一种新型腺病毒含有的细小副总裁转录单位（AD-VP）5，基于辅助。

Tags

Recombinant ParvovirusAdenovirus-derived SystemsRodent ParvovirusesPV GenomePromoters P4 And P38Non-structural Proteins (NS1 And NS2)Capsid Proteins (VP1 And VP2)Anticancer AgentsOncolytic AbilitiesOncosuppressive AbilitiesNS1 CytotoxicityTherapeutic TransgeneRecPVs VectorViral DNA AmplificationPackagingProducer Cells (HEK293T)PCMV-VP VectorReplication DefectiveOncotoxic ActivitiesAnti-cancer Agents

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