È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 01:40Flow Cell Preparation
- 02:52Magnetic Tweezers Set-up and Calibration
- 05:55Collagen Peptide Attachment to Flow Cells
- 07:00Force Proteolysis Assay
- 07:56Results: The Effect of Force on Enzymatic Proteolysis
- 08:46Conclusion
Tags
MultiplexedSingle-moleculeForceProteolysisMeasurementsMagnetic TweezersGenerationDetectionMechanical ForcesCell PhysiologyCancer MetastasisAtherogenesisWound HealingExtracellular Matrix (ECM)Enzymatically RemodelForces On ECMBiological ImportanceMedical ImportanceSingle Molecule TechniquesOptical TrappingAtomic Force MicroscopyMagnetic Tweezers (MT)Low CostHigh ThroughputPN (piconewton)Millisecond Temporal ResolutionEnzyme MechanismHighly Parallelizable AssayTrimeric Collagen PeptideMatrix Metalloproteinase 1 (MMP-1)Substrates And Proteases
