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3维建模和成像集体细胞的侵袭
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Medicina
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JoVE Journal Medicina
Modeling and Imaging 3-Dimensional Collective Cell Invasion
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3维建模和成像集体细胞的侵袭

DOI:
10.3791/3525-v

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07:08 min

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December 07, 2011

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, ,
1Strathclyde Institute for Pharmacy and Biomedical Sciences,University of Strathclyde , 2The Beatson Institute for Cancer Research

Capitoli

  • 00:05Titolo
  • 01:04Retroviral Labeling of Cells With Fluorescent Proteins
  • 02:45Inverse Matrigel Invasion Assay
  • 04:16Staining and Visualization
  • 06:07Contributions of Candidate Proteins to Collective Invasion
  • 06:50Conclusion

Summary

Traduzione automatica

Traduzione automatica

成三维的细胞外基质肿瘤细胞的侵袭模型更好地反映<em>在体内</em>比二维的蠕动检测情况。可使用矩阵入侵检测结合共焦成像与荧光标记的细胞，对领导与下面的细胞入侵模式和独特的贡献的详细信息。

Tags

ModelingImaging3-dimensionalCollective Cell InvasionCancer MalignancyInvasionMetastasisGliomaBreast CancerLung CancerMetastasis ProcessTumor CellsOrgan FailureMorbidityMortalityMolecular ProcessesProtein MediatorsDiagnosisTreatmentInvasion AssaysIn Vivo SituationTwo-dimensional Cell Motility AssaysExtracellular Matrix (ECM) Protein RemodelingTumor Cell InvasionIndividual Cells MovementCollective Invasion

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