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改良酵母双杂交系统，以确定蛋白与生长因子Progranulin的相互作用
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Biologia
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JoVE Journal Biologia
Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin
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改良酵母双杂交系统，以确定蛋白与生长因子Progranulin的相互作用

DOI:
10.3791/3562-v

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07:56 min

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January 17, 2012

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, ,
1Department of Orthopaedic Surgery,NYU Hospital for Joint Diseases, 2Department of Cell Biology,New York University School of Medicine

Capitoli

  • 00:05Titolo
  • 01:07Small Scale Transformation of Bait Plasmid
  • 02:12Bait Validation
  • 03:09cDNA Library Screening
  • 05:06X-gal Assay
  • 05:59Retransformation in the MY2H Assay
  • 06:47Isolation of Progranulin-Interacting Proteins
  • 07:32Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们已经修改了传统的酵母双杂交筛选，确定蛋白质相互作用的一种有效的遗传工具。此修改的过程明显缩短，减少了工作量，最重要的是，降​​低了误报数量。此外，这种方法是重复的，可靠的。

Tags

Modified Yeast-two-hybrid SystemProteinsInteractingGrowth FactorProgranulinPGRNGranulin Epithelin PrecursorAutocrine Growth FactorPhysiologic ProcessesDisease ProcessesInflammationHost DefenseNeurotrophic FactorPGRN GeneFrontotemporal DementiaCartilage Development And DegradationBinding Receptor(s)Modified Yeast Two-hybrid ApproachGAL4 Based 2-hybrid SystemFalse Positive ClonesReproducible And Reliable System

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