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Modifisert Gjær-Two-Hybrid System for å identifisere proteiner i samspill med Growth Factor Progranulin
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Biologia
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JoVE Journal Biologia
Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin

Modifisert Gjær-Two-Hybrid System for å identifisere proteiner i samspill med Growth Factor Progranulin

DOI:
10.3791/3562-v

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07:56 min

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January 17, 2012

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, ,
1Department of Orthopaedic Surgery,NYU Hospital for Joint Diseases, 2Department of Cell Biology,New York University School of Medicine

Capitoli

  • 00:05Titolo
  • 01:07Small Scale Transformation of Bait Plasmid
  • 02:12Bait Validation
  • 03:09cDNA Library Screening
  • 05:06X-gal Assay
  • 05:59Retransformation in the MY2H Assay
  • 06:47Isolation of Progranulin-Interacting Proteins
  • 07:32Conclusion

Summary

Traduzione automatica

Traduzione automatica

Vi har endret den konvensjonelle gjær to-hybrid screening, et effektivt genetiske verktøy for å identifisere protein interaksjon. Denne endringen forkorter markert prosessen, reduserer arbeidsmengden, og viktigst, reduserer antall falske positiver. I tillegg er denne tilnærmingen reproduserbar og pålitelig.

Tags

Modified Yeast-two-hybrid SystemProteinsInteractingGrowth FactorProgranulinPGRNGranulin Epithelin PrecursorAutocrine Growth FactorPhysiologic ProcessesDisease ProcessesInflammationHost DefenseNeurotrophic FactorPGRN GeneFrontotemporal DementiaCartilage Development And DegradationBinding Receptor(s)Modified Yeast Two-hybrid ApproachGAL4 Based 2-hybrid SystemFalse Positive ClonesReproducible And Reliable System

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