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Stem Cell Transplantation in an in vitro Simulated Ischemia/Reperfusion Model
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Medicina
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JoVE Journal Medicina
Stem Cell Transplantation in an in vitro Simulated Ischemia/Reperfusion Model

Stem Cell Transplantation in an in vitro Simulated Ischemia/Reperfusion Model

DOI:
10.3791/3575-v

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09:15 min

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November 05, 2011

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, , , ,
1Institute of Human Physiology and Clinical Experimental Research,Semmelweis University

Capitoli

  • 00:05Titolo
  • 02:20Preparing H9c2 Cardiomyoblast Cells
  • 03:44Simulated Ischemia/Reoxygenation
  • 05:25Preparing Rescue Mesenchymal Stem Cells
  • 06:21Live/Dead Staining of Co-cultivated Cells
  • 07:23Analysis with Confocal Microscopy
  • 07:47Analysis with Flow Cytometry
  • 08:36Representative Results

Summary

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We demonstrate how to set up an in vitro ischemia/reperfusion model and how to evaluate the effect of stem cell therapy on postischemic cardiac cells.

Tags

Stem Cell TransplantationIn VitroSimulated Ischemia/reperfusion ModelClinical PracticeRobust ProtocolsNew SourcesImplantable CellsCell TherapiesTreatment Process MechanismsExperimental ProtocolIschemia/reperfusion InjuryStem Cell Transplantation EfficacyH9c2 Cardiomyoblast CellsCell Culture PlatesGlucose Free MediumOxygen LevelsReperfusion SimulationDamaged Cells TreatmentLabeled Human Bone Marrow Derived Mesenchymal Stem CellsCo-cultivationLive And Dead Cells Differentiation

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