È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 01:16Cell Culture
- 01:40Amino Acid Extraction
- 02:46Amino Acid Derivatization and GC-MS Conditions
- 04:07GC-MS Data Analysis
- 05:41Representative GC-MS Analysis
- 07:06Conclusion
13 C-isotoop labeling is een nuttige techniek voor het bepalen van de cel centrale metabolisme voor verschillende soorten micro-organismen. Na de cellen zijn gekweekt met een specifiek gelabeld substraat, kunnen GC-MS metingen onthullen functionele metabole routes op basis van unieke patronen in de etikettering proteïnogene aminozuren.
Tags
Metabolic Pathway ConfirmationMetabolic Pathway Discovery13C LabelingProteinogenic Amino AcidsBiochimicaFunctional Genomics13C-assisted Metabolism AnalysisIsotopic LabelingAtom Transition PathsBiochemical NetworkTranscriptomicsProteomicsIsotopomer-assisted Analysis Of Metabolic PathwaysLabeled SubstratesMinimal MediumCarbon SourceMeasurement NoisesNon-labeled CarbonNutrient SupplementsLabeled Substrate ChoiceReaction StereochemistryIntermediate ProductsSingly Labeled Carbon SubstratesUniformly Labeled SubstratesAmino Acid Labeling PatternsGC-MS
