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确定临床抗体样品的吞噬活性
JoVE Journal
Immunologia e infezioni
This content is Free Access.
JoVE Journal Immunologia e infezioni
Determining the Phagocytic Activity of Clinical Antibody Samples
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确定临床抗体样品的吞噬活性

DOI:
10.3791/3588-v

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09:05 min

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November 30, 2011

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, , , , ,
1Massachusetts General Hospital,Ragon Institute of MGH, MIT, and Harvard, 2Thayer School of Engineering,Dartmouth College

Capitoli

  • 00:05Titolo
  • 02:01Preparing Biotinylated Antigen
  • 02:58Preparing Antigen Saturated Beads
  • 04:08Preparing Antibody Samples and Plating the Experiment
  • 05:44Flow Cytometric Analysis
  • 06:29Antigen-saturated Beads and Phagocytosed in an Antibody-dependent Manner
  • 08:24Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们目前的高通量流式细胞仪检测，以确定从临床标本中的抗原特异性抗体的吞噬活性，利用荧光抗原包被的磁珠和单核细胞表达多个Fc受体提供一个标准化的受体的使用和吞噬活性测定重现时尚任何利益抗原。

Tags

Phagocytic ActivityClinical Antibody SamplesFc ReceptorsClearancePathology Of DiseaseVariable DomainsInnate Immune CellsRecombinant Monoclonal Antibody TherapyNatural InfectionVaccinationIgG SubclassGlycosylationFunctional DifferencesAntigen-specific AntibodiesDisease StateSusceptibility To InfectionProgressionClinical OutcomeEffector FunctionEnhance InfectionFc Receptor-driven PhagocytosisImmune ComplexesTh1/Th2 Polarization Assay

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