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Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System
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JoVE Journal Neuroscienze
Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System

Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System

DOI:
10.3791/3597-v

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09:39 min

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April 15, 2012

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, , , ,
1Department of Biological Sciences,Florida Atlantic University, 2Department of Chemistry & Biochemistry,Florida Atlantic University

Capitoli

  • 00:02Titolo
  • 00:48Electrophysiology rig set-up
  • 01:18Nanoinjection set-up
  • 03:14Drosophila melanogaster preparation
  • 03:58Paired nanoinjection/electrophysiology
  • 07:35Risultati

Summary

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A rapid in vivo assay to test for neuromodulatory compounds using the Giant Fiber System (GFS) of Drosophila melanogaster is described. Nanoinjections in the head of the animal along with electrophysiological recordings of the GFS can reveal bioactivity of compounds on neurons or muscles.

Tags

NanoinjectionElectrophysiology AssayScreenBioactivityCompoundsDrosophila MelanogasterGiant Fiber SystemIn Vivo ActivityPharmacological AgentsModulatory EffectsNeuronal CircuitSmall MoleculesPeptidesMolecular TargetsGap JunctionsCholinergicNicotinic Acetylcholine ReceptorsNeuromuscular JunctionsGlutamatergicNanoliter QuantitiesIntracellular Recordings

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