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Amid brint / Deuterium Exchange & MALDI-TOF-massespektrometri Analyse af Pak2 Aktivering
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Biologia
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JoVE Journal Biologia
Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation

Amid brint / Deuterium Exchange & MALDI-TOF-massespektrometri Analyse af Pak2 Aktivering

DOI:
10.3791/3602-v

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07:15 min

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November 26, 2011

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,
1Department of Chemistry,Tunghai University, 2Department of Biochemistry,University of California, Riverside

Capitoli

  • 00:05Titolo
  • 02:14Identification of Pepsin-digested Pak2 Fragments
  • 03:34Measurement of Amide H/D Exchange
  • 05:30Time Course of Deuterium Incorporation for Inactive Pak2
  • 06:41Conclusion

Summary

Traduzione automatica

Traduzione automatica

MALDI-TOF massespektrometri blev succesfuldt udnyttet til at overvåge amid hydrogen / deuterium udveksling i protein kinase Pak2 aktivering.

Tags

Amide Hydrogen/deuterium ExchangeMALDI-TOF Mass SpectrometryProtein-protein InteractionsProtein Conformational ChangesProtein DynamicsProtein-ligand InteractionsDeuteration RatesPepsin DigestionResolutionHeteronuclear Single Quantum Coherence (HSQC)NMRAqueous SolutionMALDI-TOF DetectionHPLC/ESI-MS SystemProtein Kinase Pak2Gamma-Pak

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