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Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow

Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow

DOI:
10.3791/3609-v

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11:36 min

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January 14, 2012

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, , ,
1Department of poverty related diseases,Barcelona Centre for International Health Research, 2Confocal Microscopy Unit,University of Barcelona- Scientific and Technological Centers, 3Institució Catalana de Recerca i Estudis Avançats (ICREA)

Capitoli

  • 00:05Titolo
  • 01:52Animal Infection
  • 03:33Ex Vivo Labeling of RBCs
  • 04:30Surgical Procedures
  • 09:184D Imaging of Parasite Mobility
  • 10:12Blood Flow
  • 10:55Conclusion

Summary

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We show the method for performing intravital microscopy of the spleen using GFP transgenic malaria parasites and the quantification of parasite mobility and blood flow within this organ.

Tags

Intravital MicroscopySpleenQuantitative AnalysisParasite MobilityBlood FlowMalaria ModelsParasite-host InteractionsPre-erythrocytic StagesSkin Lymph NodesHepatocyte-derived MerosomeMerozoitesErythrocytesProtein ExportPlasmodium DevelopmentDynamic PassageLymphoid OrganInfected Red Blood CellsPlasmodium YoeliiBalb/c MiceBarrier CellsFibroblastic OriginRed Pulp

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