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流感病毒的高通量检测方法
JoVE Journal
Immunologia e infezioni
This content is Free Access.
JoVE Journal Immunologia e infezioni
High-throughput Detection Method for Influenza Virus
DOI:

10:05 min

February 04, 2012

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Capitoli

  • 00:05Titolo
  • 02:26Intra-nasal Infection of Mice with Influenza and Collection of Bronchio-Alveolar Lavage Fluid
  • 03:22Infection of MDCK Cells with Virus and Detection of Viral M2 Protein with the LI-COR Odyssey
  • 08:09Quantification of Influenza Viral Titer using Infra-red Staining and Detection
  • 09:16Conclusion

Summary

Traduzione automatica

此方法介绍了染料的红外成像系统的使用H1N1病毒的检测,在细支气管肺泡灌洗(BAL)感染小鼠的高灵敏度流体。这种方法可以在96 - 或384孔板,要求<10μL测试材料的量,并有可能并发多种病原体筛查。

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