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Ex Vivo Organotypic Corneal Model of Acute Epithelial Herpes Simplex Virus Type I Infection
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JoVE Journal Neuroscienze
Ex Vivo Organotypic Corneal Model of Acute Epithelial Herpes Simplex Virus Type I Infection

Ex Vivo Organotypic Corneal Model of Acute Epithelial Herpes Simplex Virus Type I Infection

DOI:
10.3791/3631-v

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07:55 min

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November 03, 2012

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, ,
1Department of Biochemistry and Molecular Biology,Drexel University College of Medicine

Capitoli

  • 00:00Titolo
  • 00:47Introduction
  • 02:08Materials
  • 02:36Excision of the Corneoscleral Button
  • 03:29Introduction of Cornea into Organotypic Culture
  • 04:22Infection with HSV-1 and Treatment with Experimental Drugs
  • 05:25Medium for Plaque Assays
  • 05:45DNA, RNA, Protein, or Cells from Corneal Epithelium
  • 06:48Tissue Samples for Immunohistochemistry or Immunofluorescence
  • 07:23Conclusion

Summary

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In this video article we describe the use of a new ex vivo model of acute herpes simplex virus type I corneal epithelial infection.

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Ex Vivo Organotypic Corneal ModelAcute Epithelial Herpes Simplex Virus Type I InfectionHerpes KeratitisHSV-1Cornea-derived BlindnessInfection-associated BlindnessCorneal EpitheliumCorneal StromaCorneal EndotheliumScarringThinningOpacityIn Vitro ModelIn Vivo ModelCultured MonolayersPetri DishPhysiological SystemEx Vivo ModelOrganotypically Maintained Culture

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