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制备纤维蛋白三维支架干细胞培养应用
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Bioingegneria
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JoVE Journal Bioingegneria
Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications
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制备纤维蛋白三维支架干细胞培养应用

DOI:
10.3791/3641-v

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07:04 min

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March 02, 2012

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,
1Department of Biology,University of Victoria , 2Department of Mechanical Engineering, Division of Medical Sciences,University of Victoria

Capitoli

  • 00:05Titolo
  • 01:31Day One: Making Fibrinogen Solutions and Overnight Dialysis
  • 02:57Day 2: Polymerization of Fibrin Scaffolds and Seeding of Stem Cells into Scaffolds
  • 06:01Representative Fibrin Scaffold Results
  • 06:38Conclusion

Summary

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Traduzione automatica

这项工作的具体编制三维纤维支架plutipotent干细胞培养和分化。这种支架可用于筛选各种生物化合物对干细胞的行为的影响，以及修改为包含药物输送系统。

Tags

3D Fibrin ScaffoldsStem Cell Culture ApplicationsStem Cells3D MicroenvironmentsStem Cell NicheBiomaterial ScaffoldsPolystyreneCell PolarityNaturally Derived BiomaterialsSynthetic BiomaterialsMechanical PropertiesChemical PropertiesReproducibilityNatural BiomaterialsExtracellular MatrixCell AdhesionFibrin Scaffolds

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