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体外拟态正常与异常的人造血

JoVE Journal
Bioingegneria

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JoVE Journal Bioingegneria

Ex vivo Mimicry of Normal and Abnormal Human Hematopoiesis

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体外拟态正常与异常的人造血

DOI:

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11:50 min

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April 10, 2012

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Teresa Mortera-Blanco, Maria Rende, Hugo Macedo, Serene Farah, Alexander Bismarck, Athanasios Mantalaris, Nicki Panoskaltsis

¹Department of Chemical Engineering and Chemical Technology, South Kensington campus,Imperial College London , ²Department of Hematology, Northwick Park & St. Mark’s campus,Imperial College London

Capitoli

00:05Titolo
01:55Scaffold Manufacturing and Bio-functionalization
03:29Mononuclear Cell Isolation and Scaffold Seeding
04:24In situ Cell Proliferation and Morphology: MTS Assay
05:04In situ Cell Proliferation and Morphology: SEM and Cytospins
06:25In situ Cell Proliferation and Morphology: Multiphoton Analysis
07:39Flow Cytometric Analysis of Cellular Population
08:53Representative Results
10:47Conclusion

Summary

Traduzione automatica

用脐血及白血病骨髓细胞三维培养一种造血系统。该方法的基础上使用涂有细胞外基质蛋白合成的多孔聚氨酯脚手架。这种支架是适应能力，以适应广泛的细胞。

Tags

Ex Vivo Mimicry Normal Hematopoiesis Abnormal Hematopoiesis Hematopoietic Stem Cells Bone Marrow Cellular Microenvironments Niches In Vivo Microenvironment Leukemogenesis Hematopoietic Cells Stromal Cells Cytokines 3D Bone Marrow Culture System Polyurethane Scaffold

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