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锁核酸流式细胞仪，荧光原位杂交（LNA流FISH）：细菌小RNA检测方法
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection
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锁核酸流式细胞仪，荧光原位杂交（LNA流FISH）：细菌小RNA检测方法

DOI:
10.3791/3655-v

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09:45 min

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January 10, 2012

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1Center for Bio/Molecular Science and Engineering,Naval Research Laboratory

Capitoli

  • 00:05Titolo
  • 01:25LNA Probes and Experimental Design
  • 02:31Fixation
  • 03:36Diethypyrocarbonate (DEPC) Treatment and Permeabilization
  • 04:53Hybridization and Post-hybridization Washes
  • 05:47Blocking and Staining
  • 07:59Representative Cell Aggregation and LNA flow-FISH Data
  • 09:15Conclusion

Summary

Traduzione automatica

Traduzione automatica

一种新型的高通量方法使单个细菌细胞，使用锁定的核酸探针和流式细胞仪荧光检测和小分子RNA和mRNA表达的相对定量描述<em>原位</em>杂交。

Tags

Locked Nucleic AcidFlow CytometryFluorescence In Situ HybridizationFISHNucleic Acid SequencesFlow-FISHLysate-based Nucleic Acid Detection MethodsStatistical AnalysisVariance AnalysisTelomere Length DeterminationCellular IdentificationGene ExpressionViral MultiplicationBacterial Community AnalysisDNA Oligonucleotide ProbesNucleic Acid AnalogsSensitivitySpecificityMelting Temperatures (Tm)Locked Nucleic Acid (LNA) Probes

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