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Immunology and Infection

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JoVE Journal Immunology and Infection
Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
 

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Article DOI: 10.3791/3657-v • 13:42 min • March 8th, 2012
March 8th, 2012 •
1, 2, 3, 3, 3
1Department of Microbiology and Parasitology, College of Basic Medical Sciences, China Medical University, 2Department of Obstetrics and Gynecology, College of Medicine, University of Arkansas for Medical Sciences, 3Department of Pathology, College of Medicine, University of Arkansas for Medical Sciences

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Characterizing T-cell epitopes of pathogens that cause localized infections such as human papillomavirus is a challenge because of limited number of T cells in circulation. A method is described in which rare T cells were isolated and were characterized starting with a very small number of cells.

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Interferon-gamma Enzyme-linked Immunospot Assay T-cell Epitopes Human Papillomavirus (HPV) Protocol Isolating And Characterizing Rare T Cells HPV Proteins Peptide-specific T Cells Loop Electrical Excision Procedure High-grade Squamous Intraepithelial Lesion Human Papillomavirus Type 16 (HPV 16) E6 And E7 Proteins IFN-g Enzyme-linked Immunospot (ELISPOT) Assay Synthetic Peptides Epitope Specific T Cells CD8 T-cell Isolation Kit Miltenyi Biotec
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